Isolation and characterization of ganglioside GMlb
نویسنده
چکیده
Yip (1) first demonstrated the enzymatic synthesis of a sialidase-susceptible monosialoganglioside from ganglioN-tetraosyl ceramide (GgOse&er) and CMP-sialic acid using rat brain homogenate as the source of the sialosyltransferase. Subsequently the structure of this ganglioside was characterized as IVSNeuAcGgOse4Cer, and was named G M I ~ (1, 2). GM1b ganglioside has since been recognized in various cell lines such as rat ascites hepatoma AH-7974 F (3, 4), bone marrow (5), mouse myeloid leukemia (6), and lymphocytes of C57 BL/6 mouse (7). Watanabe, Powell, and Hakomori (8) first reported its natural occurrence in the human erythrocyte membrane. Nakamura et d. (9) recently characterized GM1b containing N-glycolyl neuraminic acid as a major component from ICR mouse spleen and also tentatively identified G ~ l b containing N-acetylneuraminic acid as a minor component. Therefore, GMlb ganglioside was considered to be a natural ganglioside species present in various mammalian tissues. In this report, we describe the isolation and characterization of G ~ 1 b from normal adult human brain. Although Chou, Nolan, and Jungalwala (10) provided MATERIALS AND METHODS
منابع مشابه
Chem. Pharm. Bull. 53(12) 1551—1554 (2005)
cosphingolipids from starfish, we have isolated cerebrosides, ceramide-lactosides, sulfatides, and gangliosides having some biological activities. As a continuation of our previous studies, we recently carried out the isolation and structure elucidation of a disialo-ganglioside molecular species, LLG-3, i.e., 8-O-Me-NeuAca2→11NeuGca2→3Galb1→ 4Glcb1→1ceramides, from the starfish Linckia laevigat...
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The monosialoganglioside hematoside (GM3) is an important precursor in the synthesis of the more complex gangliosides. To obtain large quantities of GM3 for use in ganglioside biosynthetic experiments, we have devised a column chromatographic procedure for the isolation and purification of GM3 from human liver. A total ganglioside mixture was obtained from a 550 g sample of normal human liver. ...
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A slow migrating monosialosyl ganglioside with blood group H activity was isolated from human erythrocyte membranes and identified as monosialosylceramide decasaccharide. Its structure was determined by degradation with exo- and endoglycosidases, by mass spectrometric characterization of a nonasaccharide liberated by endo-beta-galactosidase, and by methylation analysis and mass spectrometry of ...
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تاریخ انتشار 2002